International Workshop on
Sustainable Fruit Growing
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Events

The International Society for Horticultural Science (ISHS), the Research Institute for Fruit Growing, Pitesti, Romania (RIFG) and the Organizing Committee for the Second Balkan Symposium on Fruit Growing (II BSFG), are pleased to invite you to attend one of the most significant events for the fruit growing sector in Balkan area, from the 5th to 7th September, 2011. Register at http://bsfg2011.icdp.ro in order to receive current information about the Symposium.

 

QUALITY OF SCIENTIFIC AND TECHNICAL SERVICES:

A. Continue and expand research area in fruit tree species biotechnology
B. Îmbunătăţireacalităţii cercetărilor laboratorului de virologie prin completarea dotării cu noi echipamente performante
C. Crearea condiţiilor de laborator necesare aplicării tehnicilor de biologie moleculară având în vedere reducerea contaminărilor

The new facilities in the laboratory will be aimed at increasing competitiveness in both directions of research in vitro plant tissue culture and virology and also development of new research topics.
Performance indicators result from investment in laboratory infrastructure:

A. Continue and expand research area in fruit tree species biotechnology
These performances are achieved with modern equipments, reliable and less electricity consuming:

Aquaria Bio Active Laminar flow hood

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Aquaria Bio Active Laminar flow hood
Use:
Equipment for biological protection during handling of biological material, cell cultures and tissue for in vitro culture.
Technical characteristics:
- Class II type A/B3;
- Vertical laminar flow class ISO 5;
- Frontal barrier;
- Hood status is monitored;
- 2 filters HEPA (main and exhaust) 99.999% effective and 6000 hours of work autonomy;
- 2 ventilators;

-Safety systems for air flow speed, window position and filters status;
- Digital display;
- Works on the principle of air recirculation 70%;
- Setting working parameters;
- Support and electrical outlet.

Flow Activa HF Aquaria hood

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Flow Activa HF Aquaria hood
Use:
Equipment for biological protection during handling of biological material, cell cultures and tissue for in vitro culture.
Technical characteristics:
- Horizontal laminar flow;
- Hood status is monitored;
- Filters HEPA 99.999% effective and 4000 hours of work autonomy;
- Safety systems for air flow speed, window position and filters status;
- Digital display;
- Works on the principle of air recirculation 70%;
- Setting working parameters;
- Support and electrical outlet.

Bidistilator Fistreem Cyclon WSC 004

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Bidistilator Fistreem Cyclon WSC 004
Use:
Automatic water still providing high grade pyrogen distilled water for in vitro culture laboratory.

Technical characteristics:
- Deionizer of water designed to provide a suitable pre - treated water to the still;
- Produces 4 lt/h of double distilled water;
- Distilled water conductivity ≤ 1mS/cm;
- Storage capacity of 30 l of distillate
- Power supply and water supply interruption protection.

Drying oven FD Binder 53 and 115

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Drying oven FD Binder 53 and 115
Use:
Sterilization of laboratory glassware, culture vessels and instruments.

Technical characteristics:
- Digital temperature setting with an accuracy of 1 °C
- Two chrome shelves standard feature:
- Temperature range: 5oC up to 300oC;
- Microprocessor temperature controller with LED display, timer function , and ramp function;
- Temperature fluctuation:(+oC): 0.3;
-  Heating up time (min.) up to 70oC (100%):7,          150oC (100%): 22, 250oC (100%): 45;

-  Nominal voltage (50/60Hz) – V: 230.

Refrigerator Sanyo MPR 513 R

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Refrigerator Sanyo MPR 513 R
Use:
Equipment for preserving reagents, stock solutions necessary for culture media.

Technical characteristics:

- Excellent cooling performance;
- Temperature control by microprocessor;
- Automatic defrosting cycle;
- Forced circulation air fan;
- Ergonomic construction;
- Alarm and safety devices;
- Effective capacity 486 l;
- 5 racks wire 20kg/raft;
- 5 sliding supports 20kg / support.

Magnetic stirrer Magnet mix TMA 2071

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Magnetic stirrer Magnet mix TMA
Use:
Designed stirring at a certain temperature of small volumes, the system continues operating.

 Technical characteristics:

- Hob stainless steel, resistant to aggressive environments;
- Adjustable speed in 80-1600 r.p.m.;
- Precise control of temp. max. 300 oC;
- Stirring capacity max. 1000 ml;
- Set stirring bars;
- Boards diameter approx. 130 mm.

Hot plates Schot SLK 2

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Hot plates Schot SLK 2
Use:
Function of heating culture media and other solutions in biological laboratories, chemical, etc.
Technical characteristics:

- Heated area 200 mm;
- Maximum temperature of plate approx. 600 oC;
- 1 l boiling water time:7 minute;
- Hot plate is glass ceramic with 25 kg load;
- Heated area 280-280 mm;
- Temperature sensor Pt-glass;
- Grip of the temperature sensor;
- Magnetic bar for standard applications AlNiCo5

Magnetic stirrer with heating Hey-tech

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Magnetic stirrer with heating Hey-tech
Use:
The instrument is designed to heat substances to a specific temperature and/or mix them through the action of a magnetic stirring rod in a vessel.
Technical characteristics:
- Speed in rpm: 100 – 1400 r.p.m;
- Heater rating: 800 W;
- Hotplate temperature range:  20-300 oC;
- Heater control: electronic;
- Hotplate material:  enameled Silumin
- Plate dimensions: 132- 132 mm;
- Max. stirring quality: 20 l;

- Magnetic stirring rod set, 3x25/50/100ml.

Technical precision balance Kern PLJ 2100- 2M

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Technical precision balance Kern PLJ 2100-2M
Use:
Determining the weight of various substances used in the preparation of culture media.
Technical characteristics:
- 5 stage filtration of vibrations;
- Maximum capacity: 2100 g;
- Readability: 0.01 g;
- Reproducibility: 0.02 g;
- Verification value: 0.1 g;
- Minimum load: 0.5 g;
- Accuracy category: II;
-Temperature packaging: 15-300 C;
- Display: LCD;
- Weighing units: mg, g, ct.

Electronic balance Kern ABJ 220- 4M

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Electronic balance Kern ABJ 220- 4M
Use:
Weighing growth regulators, micro-nutrients and vitamins necessary for preparation of culture media.
Technical characteristics:
- Internal calibration;
- Response time: 3 sec;
- RS 232 C data interface;
- GLP / ISO memory storage weighing and identification number, date and time;
- 3 stages of GLP / ISO memory storage weighing and identification number, date and time;
- 3 stages filtration of vibrations
- Weighing range (max): 220 g;
- Reproducibility; 0.1 mg;
- Precision: 0.1 mg.

pH meter 330i

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pH meter 330i
Use:
To measure and record digital pH culture media.

Technical characteristics:
- ph measuring range resolution: 0,001;
- Measuring range: -2.000.... +19.999ph,
- Temperature range:  -5.0 … +105.0 oC;
- Resolution: 0,1;
- Automatic calibration system;
- PH correction function: manual - 20 ....+ 130;
- Analog output.

Vertical steamer Raypa  AES 75

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Vertical steamer Raypa AES 75
Use:
Reliable and repeatable sterilization processes necessary for in vitro culture and virology laboratories (nutrient media and buffer solutions, pipettes, tubes, filters, plastic and glass items).
Technical characteristics:
- Capacity: 75 l;
- 10 standard individual program;
- Sterilization time: 3 -120 min.;
- Sterilization temperature:105 – 139°C;
- Safety devices for over temp., overpressure and safety valve;
- Equipped with 2 stainless steel perforated baskets and 2 non-perforated.

Stereo  Zoom Microscopes MSZ 5400

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Stereo  Zoom Microscopes MSZ 5400
Use:
Instrument for enlarged, spatial observation of small objects useful in tissue culture laboratory.

Technical characteristics:
- Built in illumination for incident  and transmitted light 6V, 15 W;
- 45º  inclined optical head with 10x flat – field eyepieces, with dioptric adjustable interocular distance 51 – 75 mm;
- Field of view diameter 32.8 - 05.1 mm;
- Zoom lenses from  0.7x 4.0x for magnification from 7x to 45 x;
- Flat – field eyepieces 20x;

- Power supply: 230V.

Barnstead Easy pure RoDi  D13321

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Barnstead Easy pure RoDi  D13321
Use:
Tap-fed water purification system designed to provide reagent grade water that exceeds ASTM Type I, and NCCLS/CAP standards for virology laboratory (molecular diagnosis).

Technical characteristics:
- Provide Type I purified water,  direct from tap water supply;
- Water resistivity:18,2 Mohmi•cm (0,055 μS/cm);
- 3,8 l/h purified water by reverse osmosis at a pressure range: 2 bar(30 psig) to 6.9 bar(100psig);
- Flow rate: 800 ml/h
- UV lamp with dual wavelength (185 and 254 nm) built;
- Storage reservoir capacity: 6,5 l equipped with an air filter that prevents contamination during storage of pure water;
- Home kit and kit prefiltration.

Glass Bead Sterilizer GRM5-1460

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Glass Bead Sterilizer GRM5-1460
Use:
Device for decontaminate microdissecting metal instruments in research laboratories. Should not be used as a substitute for traditional methods of terminal sterilization.

Technical characteristics:
- Operating temperature: 500 grade F;
- Short time sterilization: approx. 15 seconds;
- Decontamination efficiency at 233 ° C;
- Certified EC No # 991004/GERM/EMC;

 

B. Improving the quality of the virology research laboratory by complete endowment with new advanced equipment:

Active Flow Mini PCR hood

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Active Flow Mini PCR hood
Use:
Equipment for biological protection of operator and environmental for the risks that occur when working with potentially dangerous substances and avoid contamination during work.
   Useful to avoid external influences during weighing.
  Specifically designed to provide protection during sample preparation for molecular biology.

Technical characteristics:
- Protection class II;
-  Negative pressure created at the access window assure maximum operator protection;
-  Complete decontamination enclosure by irradiation with UV neon lamp germicide type 15 W;
- 99,999% 2 HEPA type filters - primary and exhaust;
-  1 exhaust carbon filter C 100/h 40;
- White light neon fluorescent type;
- Setting working parameters;
-  Support and electrical outlet.

Ultra Low Temperature Freezer Sanyo MDF

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Ultra Low Temperature Freezer Sanyo MDF
 Use:
Freezer for preservation of biological samples in molecular biology (PCR).

Technical characteristics:
- Capacity 333 l;
- Stainless steel, 3 shelves adjustable;
- 1 external door closing;
- 2 interior doors insulated with sealing;
- Refrigerant : R-404;
- Microcomputer control system for temperature;
- Thermal sensor: platinum resistance (Pt 1000 ohms);
- High temp. alarm, low temp. alarm, power failure alarm, door alarm;
- Temperature range: from -50oC to -86oC.

Ice machine Ziegra ZBE 70 - 35

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Ice machine Ziegra ZBE 70-35
Use:
Ice machine product is useful in molecular biology work in the diagnosis of viruses by PCR method.

Technical characteristics:
- Capacity/24 h: 70 kg chip ice at 20°C  and 15°C water temperature;
- Storage bin stainless steel inside and outside 40 mm PU – foam: 35 kg;
- Electricity power consumption: 0.4kW/2.5°;
- Refrigerant: 0.4kW/2.5°;
- Evaporation temperature: -14°C±2°;
- Water drainage, automatically at stop of ice production.

Distilled Fistreem Cyclon

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Distilled Fistreem Cyclon
Use:
Automatic water still for supply distilled water of good quality for use in vitro cultures laboratory.

Technical characteristics:

- Water obtained by distillation method;
- Borosilicate glass boiler;
- Flow of water 4 lt/h double distillation;
- Resistant covered in the quartz sheaths;
- PH range of 5,6-6,0;
-  Distilled water conductivity ≤ 1 mS / cm 
- Protection to stop power supply;
- Protection of water supply interruption.

Washing plates Tecan Hydro Flex Platform

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Washing plates Tecan Hydro Flex Platform
Use:
The main application is the manual and automated processing of ELISA assays and the washing of adherent cells.
 It is use for ELISA – washing of protein –arrays spotted into 96-well microplates.

Technical characteristics:
- Number of dispensing channels: up to four channels;
- Manifold types: 8 and 16 way manifold
- Display unit: liquid crystal with two rows of sixteen digits;
- Dispensing accuracy: <= 2 %;
- Dispensing uniformity: <= 4 %;
- Residual volume: <= 2 %;
- Volume of solution dispense: 50 – 3000 μl in 50 increment, for washing; 50 – 400 μl in 50 increment, for dispensing;
-Adjustable suction power;
- Automatic detection of air bubbles;

- Bottle set: 3x2, 5 l.

Centrifuge Hettich Micro 220

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Centrifuge Hettich Micro 220
Use:
For virology laboratory works (ELISA, PCR) in different stages of sample preparation.

Technical characteristics:

- Microprocessor and display with digital display for parameter values during centrifugation;
- Moisture-protected front panel;
- Programmable keys;
- Programming of rotational speed with increments of 10 rpm;
- Programming with increments of 10 centrifugal force RCF;
- Possibility of setting time centrifuge;
- Software timer (minutes / seconds);
- Possibility of storage and recall of three full programs centrifuge;
- 9 levels preselected acceleration / interruption,
- Rotor angular for PCR strips min. 18,000 RCF with a lid.

Stereo Microscope STEMI 2000 with photo camera

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Stereo Microscope STEMI 2000 with photo camera
Use:
Precision instrument for enlarged, spatial visual, photo or video observation of microscopic specimens in laboratory.
Technical characteristics:
- Illumination mode: reflected, transmitted and mixed light;
- Principle of operation: continuous zoom;
- Total magnification: 6.5X-50X
- Change magnification function and focusing: manual;
- Allow the eye examination or with the camera, having a dedicated optical output for documentation;
- Object field diameter: 35.4 mm - 4.6 mm;
- Variable cold light source, min. 12V/150W;
- Specimen field: 35.4 mm - 4,6 mm;
- Software for acquisition, archiving and analysis of images composed and digital camera control functions
Compact digital camera:
- Resolution: minimum 12Mpixeli
- Minimum 4X optical zoom
- Minimum 4X digital zoom
- Internal Memory: 32Mb minimum.

 

C.  Creating the laboratory conditions necessary to apply molecular biology techniques considering reducing contamination.

Taking into account good practice rules some of the essential aspects of contamination control program were solve by isolation particularly in the virology area and pavement the floors with epoxy resin to seamless joints, and instead of joining wall – floor concave.

Separating post PCR of pre PCR activities, the contamination potential is significantly reduced.

Flow is unidirectional from clean areas to contaminated areas, but not vice versa.

Equipments are those strictly necessary for testing activities, located and dedicated in work areas to prevent their removal and accidental cross-contamination.